During incubation, metabolism produces color changes that are either spontaneous or revealed by the addition of reagents. A bacterial suspension is used to rehydrate each of the wells and the strips are incubated. The other API system is specific for Gram positive bacteria, including Staphylococcus species, Micrococcus, species, and related organisms, and is called API-Staph.ĪPI test strips consists of wells containing dehydrated substrates to detect enzymatic activity, usually related to fermentation of carbohydrate or catabolism of proteins or amino acids by the inoculated organisms. One of the API systems is specific for differentiating between members of the Gram negative bacterial Family Enterobacteriaceae and is called API-20E. The API range introduced a standardized, miniaturized version of existing techniques, which up until then were complicated to perform and difficult to read. Presently, the API test system is manufactured by bioMérieux. It was invented in the 1970s in the United States by Pierre Janin of Analytab Products, Inc. Because of this, only known bacteria can be identified. This system is developed for quick identification of clinically relevant bacteria. The analytical profile index or API is a classification of bacteria based on biochemical tests, allowing fast identification. API 20NE Detection system after 24 hours incubation.
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